Real World Test Cases
Below are some test cases taken from commissioning experiments for the Mid-IR beamline showing real world performance available with using synchrotron light.
This experiment was done in transmission mode in D2O. The correlation between the integrated infrared and the real image can be seen very easily.
Diffraction limited infrared map of the cilium in a retinal rod cell.
Top spectrum shows inclination of rhodopsin α-helices: Micro-IR θ = 28°, XRD θ= 24°. Spectrum below shows the lipid acyl chain orientation ν=C-H θ= 45°, νCH2 θ= 61°, with average tilt for an acyl chain of 35°. The blue spectra correspond to measurements parallel to the rod axis, while red is perpendicular. The picture on the left shows the lipid ester carbonyl orientation νC=O θ = 70° (67° and 72°).
Linear dichroism measurements of intact rod cells in D2O buffer solution.
Reflection geometry measurement of the resolution comparing the performance of the Hyperion 2000 microscope using synchrotron light versus the regular globar source.
Diffraction limited maps of a 1951 USAF target
Mapping a 2.7μm thick PDMA pattern on a silicon wafer. The plot shows the absorption at 1510 cm-1, using a transmission geometry (36X objective and condenser), 3μm x 3μm upper aperture, and 1μm step size.
Measuring the system’s resolution in transmission mode
Mapping creatine crystals in brain tissue with decreasing confocal aperture size in reflection geometry. The spectra on the right show results of measuring with two different aperture sizes, as a comparison. Sample courtesy of Kathy Gough, University of Manitoba.
The effect of decreasing aperture size…